Oxygen Radicals in Biological Systems

Oxygen Radicals in Biological Systems

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Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 234 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. Key Features * Generation, detection, and characterization of oxygen radicals, chemistry, biochemistry, and intermediate states of reductio* Isolation, characterization, and assay of enzymes or substrates involved in formation or removal of oxygen radical * Methods for assessing molecular, cell, and tissue damage; assays and repair of oxidative damageThe chamber of the reader should be incubated at 37Ad, and a shaking cycle should be included between readings if ... Use of Nitro Blue Tetrazolium to Assay Superoxide Production The nitro blue tetrazolium (NBT) slide test was devised as aanbsp;...

Title:Oxygen Radicals in Biological Systems
Author: Lester Packer
Publisher:Elsevier - 1994

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